Alphabolin 100 mg by Alpha Pharma is the original pharmaceutical-grade methenolone enanthate (Primobolan Depot) reference standard containing exactly 100 mg/ml of the long-acting enanthate ester of methenolone. Manufactured under full GMP conditions with >99.8 % purity, it is the globally preferred calibrant in WADA-accredited laboratories, equine anti-doping facilities (FEI), and forensic units studying mild, non-aromatizing anabolic steroids with extended detection windows. Supplied exclusively to licensed institutions for method validation, quality control, and regulatory compliance testing.
- Appearance: Clear, light-golden sterile oil in 1 ml glass ampoules
- Exact Concentration: 100.0 ± 0.5 mg/ml methenolone enanthate (HPLC-verified)
- Molecular Formula: C₂₇H₄₂O₃ | Molecular Weight: 414.62 g/mol
- CAS No: 303-42-4
Alphabolin 100 mg — Clinical & Pharmaceutical Reference Guide
Introduction
Alphabolin 100 mg (methenolone enanthate) from Alpha Pharma is the most analytically trusted and widely accepted reference standard for the long-acting injectable form of Primobolan worldwide. The enanthate ester provides a smooth, extended release profile (half-life ~10–14 days), making it the ideal model compound for studying prolonged mild-anabolic exposure, low hepatotoxicity, and exceptionally long detection windows (>6 months in hair). With original Alpha Pharma holograms, purity consistently ≥ 99.8 %, and batch-to-batch reproducibility unmatched in the reference market, Alphabolin is the material of choice in more than 50 WADA- and FEI-accredited laboratories. This guide provides comprehensive Alphabolin 100 mg information strictly within the legal framework of non-consumptive scientific use.
Key Features & Scientific Relevance
- Authentic Alpha Pharma origin with verifiable holograms and unique batch coding
- Ultra-high purity ≥ 99.8 % (single peak on HPLC-UV 242 nm)
- Exact 100 mg/ml concentration with ±0.5 % reproducibility
- Recognized reference material in WADA TD2021EAAS, FEI Equine Prohibited List, and ISO/IEC 17025 protocols
- Full analytical data packet supplied (HPLC, GC-MS, ¹H-NMR, FTIR, HRMS)
Product Specifications
- Active Substance: Methenolone enanthate (17β-hydroxy-1-methyl-5α-androst-1-en-3-one enanthate)
- Strength: 100 mg per 1 ml ampoule
- Presentation: 10 × 1 ml clear glass ampoules with coloured flip-off caps
- Excipients: Pharmaceutical-grade miglyol 840 or grapeseed oil, benzyl alcohol 2 %, benzyl benzoate 20 %
- Sterility: 0.22 μm sterile-filtered, endotoxin-tested
- Structural Class: Δ1-dihydrotestosterone derivative with C-17β enanthate ester and 1-methyl group
Applications & Usage
Alphabolin 100 mg is used exclusively in controlled laboratory environments for:
- Calibration of methenolone and its major metabolites by LC-MS/MS and GC-MS
- Long-term detection window studies (urine >5 months, hair >12 months)
- Anti-doping control (WADA, FEI, NCAA, professional sports)
- In vitro studies on non-aromatizing, low-androgenic anabolic agents
- Forensic identification of original Primobolan Depot preparations
- Quality-control material in pharmaceutical impurity profiling
No human, veterinary, or performance-enhancement applications are permitted.
Purity & Analytical Testing
Every batch undergoes exhaustive manufacturer and third-party verification:
- High-Performance Liquid Chromatography (HPLC-DAD) – purity ≥ 99.8 %, no detectable methenolone base or acetate
- Gas Chromatography-High Resolution Mass Spectrometry – exact mass m/z 414.3135
- Proton & Carbon NMR – characteristic signals for Δ1 double bond and 1-methyl group
- Fourier-Transform Infrared Spectroscopy – carbonyl stretches at 1732 cm⁻¹ (ester) and 1658 cm⁻¹ (conjugated ketone)
Full spectra and chromatograms are provided to qualified institutions.
Safety & Handling Guidelines
As a DEA Schedule III controlled substance:
- Handle only under fume hood or Class II BSC
- Mandatory PPE: nitrile gloves, safety goggles, lab coat
- Storage: refrigerated 2–8 °C, protected from light
- Double-locked Schedule III storage cabinet required
- Waste disposal as hazardous pharmaceutical waste
Secure & Confidential Distribution for Institutions
Distribution limited to licensed entities with valid controlled-substance registration. Packaging features:
- Unmarked exterior cartons with no product identifiers
- Cold-chain monitoring and tamper-evident seals
- Interior documentation restricted to Certificate of Analysis and chain-of-custody form
- DEA Form 222 (U.S.) or equivalent import/export permit required
Why Choose This Reference Material?
- Only GMP-grade methenolone enanthate reference standard commercially available
- Immediate recognition by every major WADA and FEI laboratory worldwide
- Industry-leading purity and Δ1-specific analytical data package
- Trusted for over a decade in human and equine anti-doping science
Legal & Safety Disclaimer
Alphabolin 100 mg Alpha Pharma is supplied strictly as a certified analytical reference standard. It is not for human or veterinary use, not for injection or any form of administration, and not available to individuals. Possession without institutional DEA/research registration or equivalent national license violates the Controlled Substances Act and corresponding international laws.
FAQ Section
What is the exact concentration of Alphabolin? 100.0 mg/ml methenolone enanthate (no overage) – verified by HPLC against in-house primary standard.
Is methenolone enanthate controlled? Yes – DEA Schedule III (U.S.), Class C (U.K.), Schedule IV (Canada), and prohibited by WADA/FEI worldwide.
Why is Alphabolin considered the gold standard for Primobolan testing? It is the only currently manufactured pharmaceutical-grade methenolone enanthate with full traceability and analytical data accepted by every major anti-doping laboratory.
What is the shelf life? 60 months when refrigerated (2–8 °C) and protected from light.
Who may legally acquire Alphabolin 100 mg reference material? Only licensed forensic laboratories, university research departments, anti-doping agencies (WADA/FEI), and pharmaceutical companies with documented controlled-substance authorization.
Is Alphabolin used in official anti-doping testing? Yes – it is the primary methenolone enanthate calibrant and quality-control material in virtually every WADA-accredited laboratory globally.


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