Induject-250 by Alpha Pharma is an original pharmaceutical-grade Sustanon reference standard containing a precise 250 mg/ml blend of four testosterone esters:

• 30 mg testosterone propionate
• 60 mg testosterone phenylpropionate
• 60 mg testosterone isocaproate
• 100 mg testosterone decanoate

Manufactured under full GMP conditions with >99.5 % total ester purity, it is the globally preferred calibrant in WADA-accredited laboratories, forensic toxicology units, and academic institutions studying multi-phasic testosterone release kinetics. Supplied exclusively to licensed entities for method development, quality control, and regulatory compliance.

• Appearance: Clear, pale-yellow sterile oil in 1 ml glass ampoules
• Total Concentration: 250.0 ± 1.0 mg/ml testosterone esters
• CAS Nos: 57-85-2 / 1255-49-8 / 15262-86-9 / 5721-91-5

Induject-250 mg — Clinical & Pharmaceutical Reference Guide

Introduction

Induject-250 (Sustanon 250 mg/ml from Alpha Pharma is the most widely recognized and analytically trusted multi-ester testosterone reference material in the world. By combining four distinct testosterone esters with staggered half-lives (propionate → phenylpropionate → isocaproate → decanoate), it produces a characteristic multi-phasic pharmacokinetic profile that is essential for studying prolonged androgen exposure, ester-specific metabolism, and extended detection windows in biological matrices. With original Alpha Pharma holograms, batch-to-batch ester ratios reproducible to within 1 %, and purity routinely exceeding 99.5 %, Induject-250 remains the gold standard in anti-doping science, forensic toxicology, and clinical research. This guide provides detailed Induject-250 information strictly within the legal framework of non-consumptive laboratory use.

Key Features & Scientific Relevance

• Authentic Alpha Pharma origin with verifiable holograms and unique batch coding
• Exact 30/60/60/100 mg ester ratio confirmed by HPLC
• Purity ≥ 99.5 % total testosterone esters (third-party verified)
• Recognized reference material in WADA TD2021EAAS, TD2022MRPL, and ISO/IEC 17025 protocols
• Full analytical data package supplied (HPLC, GC-MS, ¹H-NMR, FTIR)

Product Specifications

• Active Ingredients (per 1 ml): – Testosterone propionate  30 mg – Testosterone phenylpropionate 60 mg – Testosterone isocaproate  60 mg – Testosterone decanoate  100 mg
• Total Testosterone Esters: 250 mg/ml
• Presentation: 10 × 1 ml clear glass ampoules
• Excipients: Arachis (peanut) oil, benzyl alcohol 2 %, benzyl benzoate 15–20 %
• Molecular Formulas: C₂₂H₃₂O₃ / C₂₅H₃₄O₃ / C₂₅H₃₈O₃ / C₂₉H₄₆O₃
• Structural Class: Multi-ester blend of 17β-hydroxyandrost-4-en-3-one

Applications & Usage

Induject-250 is used exclusively in controlled laboratory environments for:

• Calibration of multi-testosterone LC-MS/MS and GC-MS assays
• Study of ester-specific hydrolysis rates in vitro
• Anti-doping control (WADA, NCAA, military, workplace testing)
• Forensic identification of Sustanon-type preparations
• Pharmacokinetic modelling of blended ester systems
• Quality-control material in testosterone replacement therapy equivalence studies

No human, veterinary, or performance-related applications are permitted.

Purity & Analytical Testing

Every batch undergoes exhaustive manufacturer and independent verification:

• High-Performance Liquid Chromatography (HPLC-UV 241 nm) – exact 30/60/60/100 ratio and ≥ 99.5 % total purity
• Gas Chromatography-Mass Spectrometry (GC-MS) – characteristic ions for each ester confirmed
• Nuclear Magnetic Resonance (¹H-NMR) – full structural confirmation of all four esters
• Fourier-Transform Infrared Spectroscopy – carbonyl stretches at 1738 cm⁻¹ (esters) and 1660 cm⁻¹ (conjugated ketone)

Full chromatograms and spectra are provided to qualified institutions.

Safety & Handling Guidelines

As a DEA Schedule III controlled substance:

• Handle only in fume hood or Class II BSC
• Mandatory PPE: nitrile gloves, safety goggles, lab coat
• Storage at controlled room temperature (15–30 °C), protected from light
• Double-locked Schedule III storage required
• Broken ampoules treated as sharps and hazardous pharmaceutical waste

Secure & Confidential Distribution for Institutions

Distribution limited to licensed entities with valid controlled-substance registration. Packaging includes:

• Unmarked exterior shipping cartons
• Cold-chain monitoring and tamper-evident seals
• Interior documentation restricted to Certificate of Analysis and chain-of-custody forms
• DEA Form 222 (U.S.) or equivalent permits required

Why Choose This Reference Material?

• Exact Organon Sustanon ester ratio for authentic multi-phasic studies
• Immediate acceptance by >50 WADA-accredited laboratories worldwide
• Full analytical data package supporting regulatory audits
• Original pharmaceutical presentation ideal for authenticity verification

Legal & Safety Disclaimer

Induject-250 Alpha Pharma is supplied strictly as a certified analytical reference standard. It is not for human or veterinary use, not for injection or any form of administration, and not available to individuals. Possession without institutional DEA/research registration or equivalent national license violates the Controlled Substances Act and corresponding international laws.

FAQ Section

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What is the exact ester breakdown in Induject-250? 30 mg propionate + 60 mg phenylpropionate + 60 mg isocaproate + 100 mg decanoate per ml.

Is Induject-250 a controlled substance? Yes – all testosterone esters are DEA Schedule III (U.S.), Class C (U.K.), and equivalently restricted globally.

How is the 30/60/60/100 ratio verified? Validated HPLC-UV assay with individual ester peak integration against USP reference standards.

What is the shelf life? 5 years when stored at 15–30 °C protected from light.

Who may legally acquire Induject-250 reference material? Only licensed forensic laboratories, universities, anti-doping agencies, and pharmaceutical companies with documented controlled-substance authorization.

Is Induject-250 used in official WADA testing? Yes – it is the most commonly used Sustanon calibrant and quality-control material in WADA-accredited laboratories worldwide.