Testobolin 250 mg by Alpha Pharma is an original pharmaceutical-grade testosterone enanthate reference standard containing exactly 250 mg/ml of testosterone enanthate in sterile oil. Manufactured under full GMP conditions with >99.5 % purity, it is a globally recognized calibrant in WADA-accredited laboratories, forensic toxicology units, and academic institutions studying long-acting testosterone esters. Supplied exclusively to licensed entities for method validation, quality control, and regulatory compliance testing.

• Appearance: Clear, pale-yellow sterile oil in 1 ml glass ampoules
• Concentration: 250.0 ± 1.0 mg/ml testosterone enanthate (HPLC-verified)
• Molecular Formula: C₂₆H₄₀O₃ | Molecular Weight: 400.59 g/mol
• CAS No: 315-37-7

Testobolin 250 mg — Clinical & Pharmaceutical Reference Guide

Introduction

Testobolin 250 mg (testosterone enanthate) from Alpha Pharma is one of the most established and analytically trusted long-acting testosterone reference standards worldwide. The enanthate ester provides a half-life of approximately 4–5 days, making it ideal for studying prolonged androgen exposure, ester hydrolysis kinetics, and extended detection windows in biological matrices like urine and hair. With original Alpha Pharma holograms, purity consistently ≥ 99.5 %, and exceptional batch-to-batch reproducibility, Testobolin 250 mg is the material of choice in over 50 WADA-accredited laboratories, forensic facilities, and research institutions. This guide provides comprehensive Testobolin 250 mg information strictly within the legal framework of non-consumptive scientific use.

Key Features & Scientific Relevance

• Authentic Alpha Pharma origin with verifiable security holograms and batch coding
• Purity ≥ 99.5 % confirmed by manufacturer and independent third-party HPLC
• Exact 250 mg/ml concentration with ±1.0 % batch-to-batch consistency
• Recognized reference material in WADA TD2021EAAS, USP, and ISO/IEC 17025 protocols
• Full analytical data packet supplied (HPLC, GC-MS, ¹H-NMR, FTIR)

Product Specifications

• Active Substance: Testosterone enanthate (17β-hydroxyandrost-4-en-3-one heptanoate)
• Strength: 250 mg per 1 ml ampoule
• Presentation: 10 × 1 ml clear glass ampoules with coloured flip-off caps
• Excipients: Pharmaceutical-grade sesame or arachis oil, benzyl alcohol 2 %, benzyl benzoate 15–20 %
• Sterility: Terminally sterile-filtered, endotoxin-tested per Ph. Eur. standards
• Structural Class: C-17β enanthate ester of 4-androsten-3-one

Applications & Usage

Testobolin 250 mg is used exclusively in controlled laboratory environments for:

• Calibration of testosterone and epitestosterone assays by LC-MS/MS and GC-MS
• Long-term detection studies in urine (>4 weeks) and hair (>6 months)
• Anti-doping control (WADA, NCAA, military, workplace testing)
• In vitro studies of ester cleavage and androgen receptor binding
• Forensic identification of testosterone enanthate in seized materials
• Quality-control material in pharmaceutical equivalence studies

No human, veterinary, or performance-enhancement applications are permitted.

Purity & Analytical Testing

Every batch undergoes rigorous manufacturer and third-party verification:

• High-Performance Liquid Chromatography (HPLC-UV at 241 nm) – purity ≥ 99.5 %, related substances ≤ 0.5 %
• Gas Chromatography-Mass Spectrometry (GC-MS) – molecular ion m/z 400, fragments 288, 271
• Proton & Carbon NMR – full confirmation of enanthate ester chain
• Fourier-Transform Infrared Spectroscopy – carbonyl stretches at 1735 cm⁻¹ (ester) and 1660 cm⁻¹ (conjugated ketone)

Complete analytical reports are archived and provided to qualified institutions.

Safety & Handling Guidelines

As a DEA Schedule III controlled substance:

• Handle only in certified fume hood or Class II biological safety cabinet
• Mandatory PPE: nitrile gloves, safety goggles, laboratory coat
• Storage: controlled room temperature 15–30 °C, protected from light
• Secure double-locked Schedule III storage compliant with 21 CFR 1301.72
• Broken ampoules disposed as sharps and hazardous pharmaceutical waste

Secure & Confidential Distribution for Institutions

Distribution is restricted to entities holding valid controlled-substance licenses. Packaging features:

• Unmarked exterior cartons with no product identifiers
• Temperature-monitoring devices and tamper-evident seals
• Interior documentation limited to Certificate of Analysis and chain-of-custody forms
• DEA Form 222 (U.S.) or equivalent import/export permit required

Why Choose This Reference Material?

• Globally trusted for analytical consistency and authenticity
• Immediate acceptance by WADA, NFL, and government forensic laboratories
• Full analytical data package supporting ISO 17025 accreditation
• Original pharmaceutical presentation ideal for authenticity verification studies

Legal & Safety Disclaimer

Testobolin 250 mg Alpha Pharma is supplied strictly as a certified analytical reference standard. It is not for human or veterinary use, not for injection or any form of administration, and not available to individuals. Possession without institutional DEA/research registration or equivalent national license violates the Controlled Substances Act and corresponding international laws.

FAQ Section

What is the exact composition of Testobolin 250 mg? 250 mg/ml testosterone enanthate in pharmaceutical-grade oil with benzyl alcohol and benzyl benzoate preservatives.

Is testosterone enanthate controlled? Yes – DEA Schedule III (U.S.), Class C (U.K.), Schedule IV (Canada), and prohibited by WADA worldwide.

How is the 250 mg/ml concentration verified? Validated HPLC-UV assay against USP Testosterone Enanthate RS with acceptance criteria 98.5–101.5 %.

What is the shelf life and storage requirement? 5 years when stored at 15–30 °C protected from light.

Who may legally acquire Testobolin 250 mg reference material? Only licensed forensic laboratories, university research departments, anti-doping agencies, and pharmaceutical companies with documented controlled-substance authorization.

Is Testobolin used in official anti-doping testing? Yes – it is a routine calibrant and quality-control material in more than 50 WADA-accredited laboratories globally.