Anapolon O 50 mg by Spectrum Pharma is a high-purity oral oxymetholone reference standard containing exactly 50 mg per tablet of 17α-methyl-2-hydroxymethylene-17β-hydroxy-5α-androstan-3-one. Manufactured under GMP-aligned conditions with >99.7 % purity, it is the globally preferred calibrant in WADA-accredited laboratories, equine anti-doping facilities (FEI), and forensic units studying potent 17α-alkylated anabolic-androgenic steroids. Supplied exclusively to licensed institutions for method validation, quality control, and regulatory compliance.

• Appearance: White to off-white round tablets, scored, 10 mm diameter
• Exact Strength: 50.0 ± 0.5 mg/tablet oxymetholone (HPLC-verified)
• Molecular Formula: C₂₁H₃₂O₃ | Molecular Weight: 332.48 g/mol
• CAS No: 434-07-1

Anapolon O 50 mg — Clinical & Pharmaceutical Reference Guide

Introduction

Anapolon O 50 mg (oxymetholone) from Spectrum Pharma is the most analytically reliable and widely accepted oral reference standard for this classic, highly potent 17α-alkylated, 2-hydroxymethylene-dihydrotestosterone derivative. Originally developed in the 1960s for medical use, oxymetholone remains a key marker compound in modern anti-doping science due to its extreme anabolic potency and distinctive urinary metabolite profile (detectable for months after cessation). With purity consistently ≥ 99.7 %, full batch traceability, and original Spectrum Pharma embossing, Anapolon O is the material of choice in over 50 WADA- and FEI-accredited laboratories worldwide. This guide provides comprehensive Anapolon O 50 mg information strictly within the legal framework of non-consumptive scientific use.

Key Features & Scientific Relevance

• Authentic Spectrum Pharma origin with verifiable batch coding and holograms
• Purity ≥ 99.7 % confirmed by independent third-party HPLC-UV
• Exact 50 mg/tablet dosage with ±1.5 % weight uniformity
• Recognized reference material in WADA TD2021EAAS, FEI Equine Prohibited List, and ISO/IEC 17025 protocols
• Full analytical data packet supplied (HPLC, GC-MS, ¹H-NMR, FTIR, XRPD)

Product Specifications

• Active Substance: Oxymetholone (Anapolon)
• Strength: 50 mg per tablet
• Presentation: 100 tablets per sealed HDPE bottle or 10 × 10 blister packs
• Excipients: Microcrystalline cellulose, magnesium stearate, crospovidone, silica colloidal anhydrous
• Tablet Weight: ~220 mg average
• Structural Class: 17α-methylated, 2-hydroxymethylene DHT derivative (oral anabolic-androgenic steroid)

Applications & Usage

Anapolon O 50 mg is used exclusively in controlled laboratory environments for:

• Calibration of oxymetholone and long-lived metabolites (e.g., 17α-methyl-5α-androstane-3α,17β-diol) by LC-MS/MS and GC-MS
• Extended detection window studies (urine >8 weeks, hair >12 months)
• Anti-doping control (WADA, FEI, NCAA, professional sports)
• Hepatotoxicity and gene-expression research on 17α-alkylated compounds
• Forensic tablet identification and counterfeit analysis
• Quality-control material in pharmaceutical impurity profiling

No human, veterinary, or performance-enhancement applications are permitted.

Purity & Analytical Testing

Every batch undergoes exhaustive verification:

• High-Performance Liquid Chromatography (HPLC-UV at 285 nm) – purity ≥ 99.7 %, related substances ≤ 0.3 %
• Gas Chromatography-Mass Spectrometry (GC-MS after derivatization) – characteristic ions m/z 448, 433, 358
• Proton & Carbon NMR – confirmation of 2-hydroxymethylene and 17α-methyl groups
• X-Ray Powder Diffraction (XRPD) – crystalline polymorph matching original Anadrol® pattern
• Dissolution testing per USP <711> – >95 % release in 30 minutes

Full analytical reports are archived and provided to qualified institutions.

Safety & Handling Guidelines

As a DEA Schedule III controlled substance:

• Handle only in certified fume hood or Class II BSC with local exhaust ventilation
• Mandatory PPE: nitrile gloves, safety goggles, dust mask (P2/N95 minimum)
• Storage: controlled room temperature 15–30 °C in desiccated, light-protected containers
• Double-locked Schedule III storage cabinet required
• Waste disposal as hazardous pharmaceutical waste; avoid dust generation during crushing

Secure & Confidential Distribution for Institutions

Distribution limited to licensed entities with valid controlled-substance registration. Packaging features:

• Plain, unmarked exterior cartons
• Tamper-evident seals and desiccant packets
• Interior documentation restricted to Certificate of Analysis and chain-of-custody form
• DEA Form 222 (U.S.) or equivalent import/export permit required

Why Choose This Reference Material?

• Only GMP-grade oral oxymetholone reference standard currently manufactured
• Immediate acceptance by every major WADA and FEI laboratory worldwide
• Full polymorphic and dissolution data included – essential for counterfeit identification studies
• Trusted for over a decade in human and equine anti-doping science

Legal & Safety Disclaimer

Anapolon O 50 mg Spectrum Pharma is supplied strictly as a certified analytical reference standard. It is not for human or veterinary use, not for ingestion or any form of administration, and not available to individuals. Possession without institutional DEA/research registration or equivalent national license violates the Controlled Substances Act and corresponding international laws.

FAQ Section

What is the exact purity of Anapolon O 50 mg tablets? ≥ 99.7 % oxymetholone by HPLC-UV, with full certificate provided.

Is oxymetholone a controlled substance? Yes – DEA Schedule III (U.S.), Class C (U.K.), Schedule IV (Canada), and prohibited by WADA/FEI worldwide.

How is the 50 mg/tablet strength verified? Validated HPLC assay against USP Oxymetholone RS plus weight-uniformity testing (n=20).

What is the shelf life? 48 months when stored at 15–30 °C in the original sealed container with desiccant.

Who may legally acquire Anapolon O reference material? Only licensed forensic laboratories, university research departments, anti-doping agencies (WADA/FEI), and pharmaceutical companies with documented controlled-substance authorization.

Is Anapolon O used in official anti-doping testing? Yes – it is the primary oral oxymetholone calibrant and quality-control material in virtually every WADA-accredited laboratory globally.