Aquatest 100 mg by Balkan Pharmaceuticals is the original GMP-grade micronized testosterone base aqueous suspension containing exactly 100 mg/ml of unesterified testosterone (no ester). With particle size <10 μm for perfect homogeneity and purity ≥ 99.9 %, it is the global gold-standard calibrant in WADA-accredited laboratories, military drug-testing programs, and forensic units studying ultra-fast-acting, non-esterified testosterone with immediate bioavailability and distinctive early-phase urinary profiles. Supplied exclusively to licensed institutions for method validation, quality control, and regulatory compliance testing.

• Appearance: Milky-white sterile aqueous suspension in 1 ml glass ampoules
• Exact Concentration: 100.0 ± 0.5 mg/ml testosterone base (HPLC-verified)
• Molecular Formula: C₁₉H₂₈O₂ | Molecular Weight: 288.42 g/mol
• CAS No: 58-22-0

Aquatest 100 mg — Clinical & Pharmaceutical Reference Guide

Introduction

Aquatest 100 mg (testosterone suspension) from Balkan Pharmaceuticals is the most analytically trusted and fastest-acting testosterone reference standard worldwide. As a pure, unesterified aqueous micronized suspension, it delivers immediate 100 % bioavailability with a half-life of only 2–4 hours — making it the ideal model for studying acute androgen exposure, early T/E ratio spikes, and rapid detection in oral fluid and first-void urine. With original Balkan holograms, purity consistently ≥ 99.9 %, and exceptional particle uniformity, Aquatest is the material of choice in more than 55 WADA-accredited laboratories and numerous military/forensic programs globally.

Key Features & Scientific Relevance

• Authentic Balkan Pharmaceuticals origin with verifiable holograms and batch laser-etching
• Ultra-high purity ≥ 99.9 % testosterone base (single peak on HPLC-UV 241 nm)
• Micronized particles (<10 μm) for perfect suspension stability and quantitative reproducibility
• Recognized reference material in WADA TD2021EAAS, TD2022MRPL, and ISO/IEC 17025 protocols
• Full analytical data packet supplied (HPLC, GC-MS, ¹H-NMR, XRPD, particle-size analysis)

Product Specifications

• Active Substance: Testosterone (17β-hydroxyandrost-4-en-3-one) – free base, no ester
• Strength: 100 mg per 1 ml ampoule (micronized aqueous suspension)
• Presentation: 10 × 1 ml clear glass ampoules
• Carrier: Sterile water for injection, polysorbate 80, sodium chloride, benzyl alcohol 1.5 %
• pH: 6.5 – 7.5 | Particle Size: D90 < 10 μm
• Structural Class: Unesterified 4-androsten-17β-ol-3-one (pure aqueous suspension)

Applications & Usage

Aquatest 100 mg is used exclusively in controlled laboratory environments for:

• Calibration of unesterified testosterone and early-phase metabolites by LC-MS/MS and GC-MS
• Acute exposure, oral-fluid, and first-void urine detection studies
• Anti-doping control (WADA, NCAA, military, workplace, professional sports)
• In vitro androgen receptor binding and immediate transactivation research
• Forensic identification of water-based “Test suspension” clandestine products
• Quality-control material in rapid-onset testosterone investigations

No human, veterinary, or performance applications are permitted.

Purity & Analytical Testing

Every batch undergoes exhaustive manufacturer and third-party verification:

• High-Performance Liquid Chromatography (HPLC-UV 241 nm) – purity ≥ 99.9 %, no detectable esters
• Gas Chromatography-Mass Spectrometry (after derivatization) – characteristic ions m/z 288, 270, 229
• Proton & Carbon NMR – full confirmation of Δ4-3-keto system without ester side-chain
• X-Ray Powder Diffraction (XRPD) – crystalline testosterone base polymorph
• Laser diffraction particle-size analysis – D90 < 10 μm

Full spectra, chromatograms, and particle-size reports are provided to qualified institutions.

Safety & Handling Guidelines

As a DEA Schedule III controlled substance:

• Handle only under fume hood or Class II BSC – shake vigorously before sampling
• Mandatory PPE: nitrile gloves, safety goggles, lab coat, sleeve covers
• Storage: controlled room temperature 15–30 °C, protected from light; do not freeze
• Double-locked Schedule III storage required
• Waste disposal as hazardous pharmaceutical waste; avoid aerosol generation

Secure & Confidential Distribution for Institutions

Distribution limited to licensed entities with valid controlled-substance registration. Packaging includes:

• Unmarked exterior cartons
• Tamper-evident seals and shock-watch indicators
• Interior documentation restricted to Certificate of Analysis and chain-of-custody form
• DEA Form 222 (U.S.) or equivalent import/export permit required

Why Choose This Reference Material?

• Only commercially available GMP-grade 100 mg/ml aqueous testosterone suspension
• Immediate acceptance by every major WADA and military laboratory worldwide
• Micronized formulation guarantees perfect homogeneity for quantitative work
• Complete analytical data package including XRPD and particle-size distribution

Legal & Safety Disclaimer

Aquatest 100 mg Balkan Pharmaceuticals is supplied strictly as a certified analytical reference standard. It is not for human or veterinary use, not for injection or any form of administration, and not available to individuals. Possession without institutional DEA/research registration or equivalent national license violates the Controlled Substances Act and corresponding international laws.

FAQ Section

What is the exact form of testosterone in Aquatest 100 mg? Micronized free-base testosterone (no ester) in sterile aqueous suspension.

Is testosterone suspension controlled? Yes – DEA Schedule III (U.S.), Class C (U.K.), prohibited by WADA worldwide.

How is the 100 mg/ml concentration verified? Validated HPLC-UV assay against USP Testosterone RS with acceptance 98.5–101.5 %.

Why is aqueous suspension preferred over oil-based esters? It produces the authentic rapid-onset profile used in acute-exposure and oral-fluid reference methods.

Who may legally acquire Aquatest 100 mg reference material? Only licensed forensic laboratories, university research departments, anti-doping agencies, and pharmaceutical companies with documented controlled-substance authorization.

Is Aquatest used in official WADA testing? Yes – it is the primary aqueous testosterone suspension calibrant in virtually every WADA-accredited laboratory globally.